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Fisher Scientific superscript ii reverse transcriptase
Superscript Ii Reverse Transcriptase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+ii+reverse+transcriptase/m+mlv+reverse+transcriptase/pmc12904577-326-5-9
Average 86 stars, based on 1 article reviews
superscript ii reverse transcriptase - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Isolation:

Article Title: Isolation and Identification of Inter-Species Enterovirus Recombinant Genomes
Article Snippet: .. Total RNA was isolated from cells using a GeneJET RNA Purification Kit (ThermoFisher, Waltham, MA, USA) following 3 cycles of freeze–thawing and clarification by centrifugation at 10,000× g for 3 min. RNA was reverse transcribed at 42 °C using oligo dT, or EV68 specific primer (EV68(-)), and SuperScript II reverse transcriptase (Fisher Scientific, Loughborough, UK) as per manufacturers’ protocol. .. The region of recombination (VP1 to 2C) was amplified using virus-specific primers ( ) and Taq polymerase (NEB, Hitchin, UK) with an initial denaturing at 95 °C for 30 s, followed by 35 cycles of 95 °C for 30 s, 50 °C for 30 s and 68 °C for 1.5 min, and a final extension at 68 °C for 5 min.

Article Title: Identification of short protein-destabilizing sequences in Arabidopsis cyclin-dependent kinase inhibitors, ICKs.
Article Snippet: Plants have a family of cyclin-dependent kinase (CDK) inhibitors called interactors/inhibitors of CDK (ICKs) or Kiprelated proteins (KRPs).. ICK proteins have important functions in cell proliferation, endoreduplication, plant growth, and reproductive development, and their functions depend on the protein levels.. However, understanding of how ICK protein levels are regulated is very limited.

Purification:

Article Title: Isolation and Identification of Inter-Species Enterovirus Recombinant Genomes
Article Snippet: .. Total RNA was isolated from cells using a GeneJET RNA Purification Kit (ThermoFisher, Waltham, MA, USA) following 3 cycles of freeze–thawing and clarification by centrifugation at 10,000× g for 3 min. RNA was reverse transcribed at 42 °C using oligo dT, or EV68 specific primer (EV68(-)), and SuperScript II reverse transcriptase (Fisher Scientific, Loughborough, UK) as per manufacturers’ protocol. .. The region of recombination (VP1 to 2C) was amplified using virus-specific primers ( ) and Taq polymerase (NEB, Hitchin, UK) with an initial denaturing at 95 °C for 30 s, followed by 35 cycles of 95 °C for 30 s, 50 °C for 30 s and 68 °C for 1.5 min, and a final extension at 68 °C for 5 min.

Clarification Assay:

Article Title: Isolation and Identification of Inter-Species Enterovirus Recombinant Genomes
Article Snippet: .. Total RNA was isolated from cells using a GeneJET RNA Purification Kit (ThermoFisher, Waltham, MA, USA) following 3 cycles of freeze–thawing and clarification by centrifugation at 10,000× g for 3 min. RNA was reverse transcribed at 42 °C using oligo dT, or EV68 specific primer (EV68(-)), and SuperScript II reverse transcriptase (Fisher Scientific, Loughborough, UK) as per manufacturers’ protocol. .. The region of recombination (VP1 to 2C) was amplified using virus-specific primers ( ) and Taq polymerase (NEB, Hitchin, UK) with an initial denaturing at 95 °C for 30 s, followed by 35 cycles of 95 °C for 30 s, 50 °C for 30 s and 68 °C for 1.5 min, and a final extension at 68 °C for 5 min.

Centrifugation:

Article Title: Isolation and Identification of Inter-Species Enterovirus Recombinant Genomes
Article Snippet: .. Total RNA was isolated from cells using a GeneJET RNA Purification Kit (ThermoFisher, Waltham, MA, USA) following 3 cycles of freeze–thawing and clarification by centrifugation at 10,000× g for 3 min. RNA was reverse transcribed at 42 °C using oligo dT, or EV68 specific primer (EV68(-)), and SuperScript II reverse transcriptase (Fisher Scientific, Loughborough, UK) as per manufacturers’ protocol. .. The region of recombination (VP1 to 2C) was amplified using virus-specific primers ( ) and Taq polymerase (NEB, Hitchin, UK) with an initial denaturing at 95 °C for 30 s, followed by 35 cycles of 95 °C for 30 s, 50 °C for 30 s and 68 °C for 1.5 min, and a final extension at 68 °C for 5 min.

Reverse Transcription:

Article Title: Isolation and Identification of Inter-Species Enterovirus Recombinant Genomes
Article Snippet: .. Total RNA was isolated from cells using a GeneJET RNA Purification Kit (ThermoFisher, Waltham, MA, USA) following 3 cycles of freeze–thawing and clarification by centrifugation at 10,000× g for 3 min. RNA was reverse transcribed at 42 °C using oligo dT, or EV68 specific primer (EV68(-)), and SuperScript II reverse transcriptase (Fisher Scientific, Loughborough, UK) as per manufacturers’ protocol. .. The region of recombination (VP1 to 2C) was amplified using virus-specific primers ( ) and Taq polymerase (NEB, Hitchin, UK) with an initial denaturing at 95 °C for 30 s, followed by 35 cycles of 95 °C for 30 s, 50 °C for 30 s and 68 °C for 1.5 min, and a final extension at 68 °C for 5 min.

Article Title: Reovirus Type 3 Dearing Variants Do Not Induce Necroptosis in RIPK3-Expressing Human Tumor Cell Lines
Article Snippet: .. RNA of cell lines was used to generate cDNA, using an oligo(dT)20 primer (Enzo Life sciences, Raamsdonksveer, The Netherlands) and Superscript II reverse transcriptase (Fisher Scientific, Landsmeer, The Netherlands). .. Expression of human RIPK3 was measured by real-time quantitative PCR (qPCR), using iQ SYBR Green Supermix (Bio-Rad Laboratories, Veenendaal, The Netherlands) on a CFx connect thermocycler (Bio-Rad Laboratories, Veenendaal, The Netherlands).

Article Title: Recurrent mutations in the stress regulator Cap1 reveal a trade-off between azole resistance and oxidative stress response in Candida albicans
Article Snippet: .. cDNA was prepared using the SuperScript II Reverse Transcriptase (Fisher Scientific) according to the manufacturer’s instructions with oligo dT primers and 100 ng of RNA. cDNA was then diluted 1:10 with nuclease-free water for qPCR measurement. .. Realtime qPCR was conducted using the PowerUp SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s instructions to measure cDNA.

Article Title: Identification of short protein-destabilizing sequences in Arabidopsis cyclin-dependent kinase inhibitors, ICKs.
Article Snippet: Plants have a family of cyclin-dependent kinase (CDK) inhibitors called interactors/inhibitors of CDK (ICKs) or Kiprelated proteins (KRPs).. ICK proteins have important functions in cell proliferation, endoreduplication, plant growth, and reproductive development, and their functions depend on the protein levels.. However, understanding of how ICK protein levels are regulated is very limited.

Article Title: Reovirus Type 3 Dearing Variants Do Not Induce Necroptosis in RIPK3-Expressing Human Tumor Cell Lines.
Article Snippet: .. RNA of cell lines was used to generate cDNA, using an oligo(dT)20 primer (Enzo Life sciences, Raamsdonksveer, The Netherlands) and Superscript II reverse transcriptase (Fisher Scientific, Landsmeer, The Netherlands). ..

Article Title: Late-Stage Glioma Is Associated with Deleterious Alteration of Gut Bacterial Metabolites in Mice
Article Snippet: Initial tissue disruption and homogenization were performed in the lysis buffer using Precellys homogenizer (Ozyme) for 2 × 15 s with “back-and-forth movement” at a frequency of 6500/min. .. Following RNA extraction, reverse transcription was performed using the Superscript II reverse transcriptase (Fisher Scientific) and random hexamers. ..

Real-time Polymerase Chain Reaction:

Article Title: Recurrent mutations in the stress regulator Cap1 reveal a trade-off between azole resistance and oxidative stress response in Candida albicans
Article Snippet: .. cDNA was prepared using the SuperScript II Reverse Transcriptase (Fisher Scientific) according to the manufacturer’s instructions with oligo dT primers and 100 ng of RNA. cDNA was then diluted 1:10 with nuclease-free water for qPCR measurement. .. Realtime qPCR was conducted using the PowerUp SYBR Green Master Mix (Applied Biosystems) according to the manufacturer’s instructions to measure cDNA.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Identification of short protein-destabilizing sequences in Arabidopsis cyclin-dependent kinase inhibitors, ICKs.
Article Snippet: Plants have a family of cyclin-dependent kinase (CDK) inhibitors called interactors/inhibitors of CDK (ICKs) or Kiprelated proteins (KRPs).. ICK proteins have important functions in cell proliferation, endoreduplication, plant growth, and reproductive development, and their functions depend on the protein levels.. However, understanding of how ICK protein levels are regulated is very limited.

Synthesized:

Article Title: Identification of short protein-destabilizing sequences in Arabidopsis cyclin-dependent kinase inhibitors, ICKs.
Article Snippet: Plants have a family of cyclin-dependent kinase (CDK) inhibitors called interactors/inhibitors of CDK (ICKs) or Kiprelated proteins (KRPs).. ICK proteins have important functions in cell proliferation, endoreduplication, plant growth, and reproductive development, and their functions depend on the protein levels.. However, understanding of how ICK protein levels are regulated is very limited.

other:

Article Title: Consequences of combined exposure to thermal stress and the plasticiser DEHP in Mytilus spp. differ by sex.
Article Snippet: Little is known about the combined effect of environmental factors and contaminants on commercially important marine species, and whether this effect differs by sex.. In this study, blue mussels were exposed for seven days to both single and combined stressors (i.e., +3 C elevated temperature and two environmentally relevant concentrations of the plastic softener DEHP, 0.5 and 50 μg/l) in a factorial design.. Males were observed to be more sensitive to high temperature, demonstrated by the significant increase in out-of-season spawning gonads and higher gene expression of the antioxidant catalase and the estrogen receptor genes.

Article Title: Sex and gametogenesis stage are strong drivers of gene expression in Mytilus edulis exposed to environmentally relevant plasticiser levels and pH 7.7
Article Snippet: Then, cDNA templates were synthesised using 200 units of InvitrogenTM SuperScriptTM II Reverse Transcriptase (Fisher Scientific, Loughborough, UK).

RNA Extraction:

Article Title: Late-Stage Glioma Is Associated with Deleterious Alteration of Gut Bacterial Metabolites in Mice
Article Snippet: Initial tissue disruption and homogenization were performed in the lysis buffer using Precellys homogenizer (Ozyme) for 2 × 15 s with “back-and-forth movement” at a frequency of 6500/min. .. Following RNA extraction, reverse transcription was performed using the Superscript II reverse transcriptase (Fisher Scientific) and random hexamers. ..



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